Profile of Carbapenemase Enzyme Encoding Genes in bacterial strains from a Teaching Hospital in Juiz de Fora-MG
Objectives: To describe genes that generate resistance to Carbapenems in bacteria isolated from a teaching hospital, observing epidemiological and genetic aspects related to their resistance. Methods: Cross-sectional study of 37 bacterial strains obtained from September 2017 to October 2018, in a teaching hospital in Juiz de Fora - MG, previously classified as multidrug-resistant by the criteria of the Clinical and Laboratory Standards Institute (CLSI). Such strains were submitted to the search for resistance genes - blaKPC, blaNDM and blaOXA-48 - through Polymerase Chain Reaction. The species, microbiological material, resistance gene, sex and age of the individuals in the samples were identified. Results: There was a balance in the distribution of sex between the samples and the mean age of the individuals was 63.86 years. The species Klebsiella oxytoca was identified in 1 sample (2.7%), Enterobacter cloacae in 1 sample (2.7%) and Klebsiella pneumoniae in 35 samples (94.6%). The blaKPC gene was detected in 81.1% of the samples, while blaNDM was not detected in the strains of this study. Even though blaOXA-48 was not tested in all samples, it was positive in 17% and, in addition, 8% of individuals had no resistance genes detected in their bacterial strains. Conclusion: Bacteria with genes encoding resistance to carbapenems and, mainly, blaKPC strains, circulate widely, leading to reduced therapeutic options and increased risk of serious infections. Therefore, the prudent choice of antimicrobials combined with prevention strategies and hospital surveillance should be prioritized.
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