Profile of Carbapenemase Enzyme Encoding Genes in bacterial strains from a Teaching Hospital in Juiz de Fora-MG
DOI:
https://doi.org/10.30968/rbfhss.2022.133.0820Abstract
Objectives: To describe genes that generate resistance to Carbapenems in bacteria isolated from a teaching hospital, observing epidemiological and genetic aspects related to their resistance. Methods: Cross-sectional study of 37 bacterial strains obtained from September 2017 to October 2018, in a teaching hospital in Juiz de Fora - MG, previously classified as multidrug-resistant by the criteria of the Clinical and Laboratory Standards Institute (CLSI). Such strains were submitted to the search for resistance genes - blaKPC, blaNDM and blaOXA-48 - through Polymerase Chain Reaction. The species, microbiological material, resistance gene, sex and age of the individuals in the samples were identified. Results: There was a balance in the distribution of sex between the samples and the mean age of the individuals was 63.86 years. The species Klebsiella oxytoca was identified in 1 sample (2.7%), Enterobacter cloacae in 1 sample (2.7%) and Klebsiella pneumoniae in 35 samples (94.6%). The blaKPC gene was detected in 81.1% of the samples, while blaNDM was not detected in the strains of this study. Even though blaOXA-48 was not tested in all samples, it was positive in 17% and, in addition, 8% of individuals had no resistance genes detected in their bacterial strains. Conclusion: Bacteria with genes encoding resistance to carbapenems and, mainly, blaKPC strains, circulate widely, leading to reduced therapeutic options and increased risk of serious infections. Therefore, the prudent choice of antimicrobials combined with prevention strategies and hospital surveillance should be prioritized.
Downloads
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2022 Authors
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
The authors hereby transfer, assign, or otherwise convey to RBFHSS: (1) the right to grant permission to republish or reprint the stated material, in whole or in part, without a fee; (2) the right to print republish copies for free distribution or sale; and (3) the right to republish the stated material in any format (electronic or printed). In addition, the undersigned affirms that the article described above has not previously been published, in whole or part, is not subject to copyright or other rights except by the author(s), and has not been submitted for publication elsewhere, except as communicated in writing to RHFHSS with this document.
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License (CC-BY-NC-ND) that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Serlf-archiving policy
This journal permits and encourages authors to post and archive the final pdf of the articles submitted to the journal on personal websites or institutional repositories after publication, while providing bibliographic details that credit its publication in this journal.
